Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc HEPES Buffer (1 M, pH 7.0) - 500ml
The HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] Buffer is a versatile Good's zwitterionic buffering agent widely used in various applications in biochemistry and molecular biology. It maintains a stable pH in the range of 6.8 to 8.2, making it ideal for various applications at physiological pH, including cell culture, protein purification, isoelectric focusing, enzyme studies, bioprocessing, and live cell imaging.
Sigma Aldrich Fine Chemicals Biosciences CAPS >=99% | 1135-40-6 | MFCD00003837 | 6X100G
CAPS >=99% | Purity: >=99% | Mol Wt: 221.32 | 1135-40-6 | MFCD00003837 | 6X100G
Research Products International Corp Tricine-SDS Sample Buffer 2X Solution, 50 ML
2X Formulation:
SDS: 8%
Glycerol: 20%
Coomassie Blue: 0.01%
Phenol Red: 0.01%
Research Products International Corp TES, Sodium Salt, 25 G
TES (N-[Tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid) Sodium Salt is a commonly used buffering agent in life science research. It helps to maintain a stable pH environment in biological experiments, such as cell culture, protein purification, and enzyme assays.
TES Sodium Salt is advantageous due to its ability to resist changes in pH over a wide range of temperatures, making it suitable for various experimental conditions.
Additionally, its low UV absorbance and minimal interference with biological molecules make it a preferred choice in many biochemical applications
Research Products International Corp TAPS 1 KG
pKa(25°:C): 8.4. The working pH range in aqueous solution is 7.7-9.1.
Research Products International Corp Manganese Chloride 1M Solution, 100 ML
Manganese Chloride is used for dC and dG tailing of DNA.
Sigma Aldrich Fine Chemicals Biosciences Hepes|7365-45-9 | MFCD00006158 | 100g
Hepes | Purity: 99.5% (titration) | MW: 238.3 | 7365-45-9 | MFCD00006158 | 100g
Sigma Aldrich Fine Chemicals Biosciences ACES >=99.0% (titration) | 7365-82-4 | MFCD00008030 | 100G
ACES >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 182.2 | 7365-82-4 | MFCD00008030 | 100G
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid | 60-00-4 | MFCD00003541 | 5kg
Ethylenediaminetetraacetic acid | Purity: ≥98.5%, powder | MW:292.24 | 60-00-4 | MFCD00003541 | 5kg
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, >=98.0% | 6976-37-0 | MFCD00002853 | 500G
BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, >=98.0% | Purity: >=98.0% | Mol Wt: 209.24 | 6976-37-0 | MFCD00002853 | 500G
Sigma Aldrich Fine Chemicals Biosciences MOPS hemisodium salt >=99% (titration) | 117961-20-3 | 250G
MOPS hemisodium salt >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 220.25 | 117961-20-3 | 250G
Boston Bioproducts Inc Tris-Acetate-EDTA Buffer, TAE (50X) - 1L
The Tris-Acetate-EDTA (TAE) Buffer is a pH regulator and chelator often used in molecular biology techniques such as agarose gel electrophoresis. Supporting a pH range of 8.1-8.4, the TAE Buffer combines tris base, acetic acid, and EDTA (Ethylenediaminetetraacetic Acid) to form a solution efficient in separating nucleic acids as a running buffer, or as a component of agarose gels. This Buffer is prepared in a 50X stock solution, and can be diluted to ideal concentrations.
ILEX LIFE SCIENCES LLC GELATIN SOLUTION 0.1 500ML
PAN-Biotech Gelatin Solution 0.1% in DPBS, w/o: Ca, Mg (500 ml). DESCRIPTION: PAN-Biotech Gelatin Solution 0.1% in DPBS, w/o: Ca, Mg for laboratory research applications. Gelatin is a heterogenous mixture of water soluble proteins of high average molecular weight present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, or bones in water. APPLICATION: Applications using gelatin include coating cell culture plates to improve cell attachment for a variety of cell types, addition to PCR to stabilize Taq DNA Polymerase, and use as a blocking agent in Western Blotting, ELISA, and immunohistochemistry. In bacteriology, gelatin can be used as a component of culture media. STORAGE: +2-8°C. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany.
Sigma Aldrich Fine Chemicals Biosciences HEPES, 7365-45-9, MFCD00006158, 500g
Empirical Formula (Hill Notation) C8H18N2O4S, ≥99.5% (titration), Molecular Weight 238.30, Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)